功劳去火胶囊中多种指标性成分的同时测定
【摘要】 目的:建立高效液相色谱法同时测定功劳去火胶囊中栀子苷、黄芩苷、盐酸小檗碱、汉黄芩苷、黄芩色谱柱素、汉黄芩素(AgilentDurashellRP)为流动相,梯度洗脱,流速个成分的含量。方法:样品以用-1磷酸二氢钾溶液(乙醇回流提取4.6mm·mLmin254nm个成分的线性范围及相关系数分别为60.003~0.178mg0.001~0.045mg批功劳去火胶囊中每粒分别含栀子苷、黄芩苷、盐酸小檗碱、汉黄芩苷、黄芩素、汉黄芩素1.0。结果:栀子苷、黄芩苷、盐酸小檗碱、汉黄芩苷、黄芩素、汉黄芩素),0.010~0.623mg0.002~0.100mgRSD),0.001~0.081mg);其加样回收率在mLmL0.40%~1.7%0.002~0.132mgmL=0.999595.5%~104.9%=0.99951.000之间;-1(r=-1(r=1.0001.000-1(r-1(r=·mL-1(r3hpH3.00-0.05molmL